Integration of Tg construct into ATF-2 gene. (A) Schematic representation of WT and Tg ATF-2 allele with Tg construct integration. White vertical boxes represent exons, and the arrowheads (a1, a2, b1, and b2) represent primers amplifying the junction sequences, parts of which are shown. Atf2 genomic sequence information was obtained from C57BL/6J mouse BAC clone RP23-14607 (accession number AL844581.7), and granzyme A genomic sequence information was obtained from C57BL/6J mouse chromosome 13 genomic contig (accession number NT_039590). (B) PCR products amplified by the indicated primer sets (panel A) are present only in Tg genomic DNA samples. WT DNA did not prime amplification. (C) RT-PCR of abnormal Atf2 transcripts in Tg NK cells. RT-PCR products from the indicated RNA samples are shown. Primers were derived from intron 10 (c1) and exon 12 of Atf2 (c2) (A). The c1:c2 product represents RNA, which has spliced out intron11. Primers from HPRT (bottom panel) indicate equivalent template availability. RNA was prepared from LAK cells of indicated mice or from EL-4 cell line.