Effect of hyperhomocysteinemia on leukocyte adhesion. (A) Plasma Hcy levels. n indicates number of mice; Diet, hyperhomocysteinemic diet. Data are presented as mean ± SEM. (B-C) Seven venules of 200 to 300 μm in diameter from 4 WT mice on chow, 7 venules from 4 WT, and 9 venules from 5 Cbs^+/– on Diet were analyzed. (B) Leukocyte rolling velocity. Cumulative histogram of rolling velocities allows direct comparison of distribution among groups; number of leukocytes analyzed: WT (538), WT/Diet (339), and Cbs^+/–/Diet (497). (C) Number of leukocytes adhering to the endothelium (> 20 seconds) over a 5-minute period. Each point represents a single venule. Leukocyte rolling velocities and number of adhering leukocytes were significantly different between Cbs^+/–/Diet and WT, as well as Cbs^+/–/Diet and WT/Diet (P < .05).