The mAbs directed against annexin A2 and S100A10 inhibit the plasmin-induced monocyte chemotaxis. (A) Effects of mAbs on the plasmin-induced monocyte chemotaxis. (B) Effects of mAbs on the fMLP-induced monocyte chemotaxis. Monocytes, nontreated (control, plasmin, and fMLP) or pretreated for 20 minutes with either mouse IgG or anti-annexin A2, anti-S100A10 (each at 10 μg/mL), or anti-PAR1 (WEDE15, 50 μg/mL) antibodies. Monocytes were allowed to migrate across a polycarbonate membrane (pore size, 5 μm) toward 0.43 CTA U/mL plasmin or 10 nM fMLP for 90 minutes. Cells on the membranes were fixed, stained, and counted microscopically. Data are presented as number of migrated cells per high-power oil immersion field (× 1000). ^**P < .01 versus IgG control. Results are the mean ± SEM of 5 independent experiments each.