Down-regulation of the annexin A2 and S100A10 expression inhibits the plasmin-induced monocyte chemotaxis and TNF-α release. (A) Antisense ODNs down-regulate the expression of annexin A2 and S100A10. After 48 hours of pretreatment with either sense (S) or antisense (AS) ODNs followed by a 12-hour recovery period monocyte cell lysates were resolved on 15% Tricine-SDS-PAGE and immunoblotted with mAbs against annexin A2 and S100A10. Actin served as loading control. (B) Effects of the ODN pretreatment on the plasmin-induced monocyte chemotaxis. Monocytes were pretreated for 48 hours with sense or antisense ODNs directed against either annexin A2 or S100A10, followed by a 12-hour recovery period. Monocytes were allowed to migrate across polycarbonate membranes (pore size, 5 μm) toward 0.43 CTA U/mL plasmin for 90 minutes. ^**P < .01 versus sense controls. (C) Effects of the ODN pretreatment on the fMLP-induced monocyte chemotaxis. Monocytes were allowed to migrate across polycarbonate membranes (pore size, 5 μm) toward 10 nM fMLP for 90 minutes. Chemotaxis data are presented as number of migrated cells per high-power oil immersion field (× 1000). Results are mean ± SEM of 4 independent experiments each. (D) Effects of the ODN treatment on the plasmin-induced TNF-α release by monocytes as analyzed by ELISA. Monocytes were incubated for 4 hours with 0.43 CTA U/mL plasmin; nd indicates not detectable. ^**P < .01, antisense versus sense. Results are the mean ± SEM of 5 independent experiments.