Equal DNA binding by GATA-1–ER and acetylation defective GATA-1(NC)–ER. EMSA with an α-globin–derived GATA site with nuclear extracts from G1E cells expressing GATA-1–ER and GATA-1(NC)–ER. As control, extracts from mouse erythroleukemia (MEL) cells and parental G1E cells were included. Anti–GATA-1 antibodies were added where indicated.