Pt1-LCL^tetcells have diminished expression of CD23 protein and transcripts with LMP1 activation. (A) Cell surface expression of CD23. pt1-LCL^tet and control LCL^tet cells were cultured continuously in the presence of LMP1 (+tet, outlined peak) or in the absence of LMP1 (–tet, shaded peak) for 4 days. Cells were then stained with anti-CD23 and analyzed by flow cytometry. (B) Relative transcript levels of CD23a and CD23b. RNA from pt1-LCL^tet cells (▪) and control LCL^tet cells (D11: □; C2: ▦) was reverse-transcribed and analyzed by quantitative real-time PCR with probe sets specific for CD23a and CD23b. Note the scale difference of the y-axis. Results are expressed as relative transcript amounts and standard deviation of 3 independent experiments.