Detection of reduced c-Rel protein expression that is not related to transcript levels. (A) Protein expression of c-Rel. Whole-cell lysate from LCL^tet cells, cultured in the presence of tet, was analyzed by Western blot and probed with antibodies against c-Rel and actin, which served as an internal loading control. (B) Nuclear translocation of c-Rel with LMP1 stimulation. Nuclear extract from LCL^tet cells, cultured in the presence (+tet) or absence (–tet) of LMP1 was also analyzed for c-Rel expression by immunoblot. (C) Relative transcript levels of c-Rel. RNA from pt1 and control LCL^tet cells was reverse-transcribed and analyzed by qPCR with probes specific for c-Rel. Results are expressed as relative transcript amounts and standard deviation of 2 independent experiments.