pH60/Surv vaccine induces changes in expression of lymphocyte homing receptors and lymphocyte distribution in Peyer patches. Lymphocytes were isolated from Peyer patches and analyzed by flow cytometry. (A) Histograms depict homing receptor expression of lymphocytes isolated from Peyer patches of pBud control (shaded area) or pH60/Surv-vaccinated (black line) mice. (Top panels) Gated on CD3⁺CD4⁺ cells. (Bottom panels) Gated on CD3⁺CD8⁺ cells. Experiment was repeated once with similar results. (B) Percentage of different lymphocyte populations in Peyer patches. indicates pBud control mice; ▪, pH60/Surv-vaccinated mice. ^*P < .05 compared with the pBud control group of mice. Experiment was repeated 3 times with similar results.