SOX4 transactivates EVI1 via AKV LTR. (A) Quantitative PCR analysis of Evi1 expression in 32Dwt18 cells. Two different Sox4-transduced clones are compared with 1 vector-transduced line and parental cells. mRNA levels are expressed as ratio to β-actin. (B) Effect of shRNA for SOX4 on mRNAs for Sox4 (top) and Evi1 (bottom) is assessed by quantitative PCR. (C) Cotransfection studies in NIH 3T3 cells using a AKV-CAT reporter transfected with increasing amounts of Sox4 expression plasmid.