Figure 5.
Maintenance of the viral genome in myeloid progenitor cells infected with either viable or UV-inactivated virus. Lysates of myeloid progenitor cells harvested at 3, 6, 12, 24, 72, and 120 hours after infection with either HCMV strain TownevarRIT3 (MOI = 3) or its UV-inactivated equivalent were serially diluted such that each reaction contained between 1 and 500 cell equivalents, and were analyzed by PCR for 40 cycles with primers IEP3C and IEP4BII. The dilution containing the lowest number of cell equivalents at which HCMV DNA was detected following infection with viable (▪) or UV-inactivated virus (□) was plotted against time after infection.