Real-time polymerase chain reaction gene expression analysis using TaqMan of RNA isolated from tissues of neonatal mice treated for 5 days with mFlt(1-3)-IgG, G6-23-IgG, or control-IgG, respectively. Genes analyzed include VEGFR-1, VEGFR-2, and PECAM/CD31 and VEGF-A, VEGF-B, and PlGF for the ligands. Data represent mean ± standard deviation of 5 RNA isolates per treatment group. Probe/primer sequences and relative RNA units (RRUs) for murine GAPDH are calculated as described previously.21 One relative RNA unit corresponds with expression levels in pooled RNA from the liver, lung, and kidney. The analysis of variance (ANOVA) between groups program was used for statistical analysis. SK indicates skeletal. *P < .05, **P < .005 relative to control treatment.
