Protein purification and Western blotting of recombinant proteins. Top: SDS-PAGE and Coomassie blue staining. Middle: Western blot using a mouse monoclonal antibody to human TF. Bottom: Western blot using mouse monoclonal antibody to His₆. M indicates molecular weight marker. Lanes 1 to 5 are identical in panels A, B, and C: lane 1, sTF-annexin V; lane 2, sTFAA-annexin V; lane 3, annexin V; lane 4, sTF; and lane 5, recombinant TF.¹⁸  Lane 6, panel A: bovine serum albumin; panels B and C: recombinant TF (Innovin; Dade Behring).