PKCϵ modulates Bcl-2 levels. (A) CD34-derived erythroblasts were cultured with IL-3, SCF, and EPO up to 14 days and total Bcl-2, PKCϵ, and Bax protein expression levels were monitored at the indicated times by Western blot. (B) Western blot of Bcl-2 in TF-1 and HeL cell lines in the presence (+) or absence (–) of PKCϵ-specific siRNA. (C) Western blot of Bcl-2 in TF-1 and HeL cell lines overexpressing wild-type (ϵ) or mutated (ϵm) PKCϵ.