Activation of the hemangioblastic and hematopoietic developmental programs is accelerated and enhanced in response to mMix. Quantitative real-time RT-PCR analysis of gene expression in i-Mix EBs cultured with (▪) or without (▦) DOX. Markers of the hemangioblast and genes involved in hematopoiesis were activated in response to induced mMix. Expression was normalized to that of a housekeeping gene (for this figure, Gapdh) and expressed as a ratio. For additional details, see Figure 2.