Lymphokine production in CD4+ cells cultured under Th-0 conditions. Naive CD4⁺ CD62L^high T cells were isolated by cell sorter from the spleens of wild-type (WT) and transgenic (TG) littermate mice and stimulated with anti-CD3/anti-CD28 Abs. (A) Protein extracts from freshly naive CD4⁺ T cells (0 hours) and activated T cells (3 hours) were processed for immunoblot analysis using anti-GILZ Ab. The same extracts were reprobed with anti–β-tubulin Ab. (B) The culture supernatants were collected at 24 and 48 hours and the cytokine concentrations were determined by ELISA. Bars represent the mean values ± 1 SEM of 3 independent experiments. ^*P < .05 represents significant differences of TG vs WT mice.