More severe bleomycin-induced lung injury and diminished DTH response in TG mice. (A) TG and WT mice were given bleomycin as described in “Materials and Methods.” (Ai) Lung water content, calculated by subtracting dry weight from wet weight. (Aii) MPO activity was evaluated 3 weeks after the last bleomycin injection. ^**P < .01 column 3 vs columns 1 and 2 (sham group); ^*P < .05 column 4 vs column 3 in panel Ai-Aii graphs. (Aiii), body weight gain of sham TG vs bleomycin-treated WT and TG mice. Data are expressed as mean ± 1 SEM from 6 mice for each group. ^*P < .05 bleomycin-treated TG vs bleomycin-treated WT. (Aiv), histologic evaluation by light microscopy of hematoxylin/eosin-stained lung section of sham WT (top left panel), sham TG (top right panel), bleomycin-treated WT (bottom left panel), and bleomycin-treated TG (bottom right panel) shows more interstitial injury and cellular infiltration in TG than in WT mice. Original magnification: × 125; insets, × 500. Images were captured with a Leitz Dialux 22 microscope with a 100×/1.25 NA oil objective (inserts were taken with a 200×/2.5 NA objective) and Axiocam Image System camera and software. The figure is representative of at least 3 independent experiments. (B) Ratios of footpad swelling of OVA-injected over PBS-injected footpads in WT mice (n = 7) and TG mice (n = 6) 24 hours after rechallenge OVA injection. Data are the mean values ± 1 SEM. ^***P < .001.