Mucosal prime-boost SHIV vaccine strategy induces CL-10–specific CD8 CTLs in mucosal and systemic lymphoid tissues. (A) Number of CL-10–specific tetramer-positive cells in colonic lamina propria. (B) Number of CL-10–specific tetramer-positive cells in peripheral blood. (C) Number of CL-10–specific cells secreting perforin in peripheral blood by ELISPOT assay. Individual data points for macaques in group 1 are color-coded as red; group 2, green; group 3, blue; and group 4, black.