Reduced MAYP expression in Mayp^Lp/Lp macrophages. SDS-PAGE and Western blot analysis of MAYP expression in NP-40 cell lysates (A) and anti-MAYP immunoprecipitates (B) prepared from BMMs of Mayp^Lp/Lp and WT mice. (C) Similar levels of expression of MAYP mRNA in BMMs of Mayp^Lp/Lp and WT mice determined by quantitative RT-PCR (± SD, triplicate assays, 2 mice per genotype). (D) LPS stimulation for 4 or 24 hours fails to elevate MAYP expression in Mayp^Lp/Lp BMMs above the level of its expression in unstimulated WT BMMs. (E) Peak response of MAYP expression to LPS follows peak responses of the proinflammatory cytokines IL-1β and TNF-α. β-Actin is the loading control.