HIF-1α activity in lymphoblastoid cell lines. (A) Samples from 2 control subjects (Con), 2 healthy heterozygotes (P09 and P11), and 2 homozygotes (P12 and P22) were cultured, and nuclear extracts were assayed for HIF-1α activity with the TransAM HIF-1 kit (Active Motif) and (B) with immunoblotting with the antibody in the TransAM HIF-1 kit. Error bars indicate 2 SDs.