HIF-1α–dependent expression in lymphoblastoid cell lines and reticulocytes. (A) Total RNA from lymphoblastoid cells of 2 control subjects (Con) and 2 homozygote patients (P12 and P22). Kidney total RNA (KR) was used as a positive control. Expression of the actin gene served as a control. (B) Total RNA from the reticulocytes of a control and a homozygote patient (P12) were used as starting material. (C) Immunoblotting analysis of aldolase and GAPDH in cellular extracts of 2 control subjects and 2 homozygotes (P12 and P22). See “Patients, materials, and methods” for further details.