Wogonin attenuates TNFα-induced NF-κB activity. (A) J-Luc-κB cells were treated with or without TNFα in the presence or absence of different amounts of wogonin as indicated. NF-κB activities were determined by luciferase assays after 8 hours of treatment. Data are representative of 3 independent experiments in triplicate assays. Error bars indicate SD. (B) Western analysis of TNFα-induced NF-κB activation in the presence and absence of 100 μM wogonin. (C) Kinetic analysis of wogonin-mediated suppression of TNFα-induced phosphorylation of IκB. J-Luc-κB cells were preincubated with 100 μM wogonin for 30 minutes and then stimulated with 50 ng/mL TNFα for 0.5 to 4 hours as indicated.