NAC does not suppress wogonin-mediated apoptosis. (A) CEM cells were treated with either 20 μM wogonin or 5 μM parthenolide in the presence or absence of different concentrations of NAC as indicated. After 24 hours of treatment, apoptotic cells were quantified by FSC/SSC FACS analysis. (B) CEM cells were treated with a combination of TNFα (10 ng/mL) and wogonin (20 μM) in the presence or absence of NAC. Apoptotic cells were determined after 24 hours of treatment as described in panel A. Data show 1 representative experiment (in triplicate assays) of 3 independent experiments. Error bars indicate SD of triplicate assays.