Wogonin does not sensitize normal T cells to TNFα-induced apoptosis. (A) Freshly isolated peripheral blood T cells (day 0), T cells after 16 hours of PHA activation (day 1), or PHA-activated cells further cultured in the presence of IL-2 for 5 days (day 6) were treated with 100 ng/mL TNFα in the presence or absence of 100 μM wogonin. Apoptotic cell death was determined by FSC/SSC after 48 hours of treatment. Results in duplicate are representative of 3 healthy donors. Jurkat cells were treated with TNFα and wogonin as a control. (B) Day 0 and day 6 T cells were treated with wogonin in combination with different concentration of His- or LZ-TRAIL for 48 hours. Jurkat cells were treated with TRAIL and wogonin as a control. Data show 1 representative experiment (in triplicate assays) of 3 independent experiments. Error bars indicate SD of triplicate assays.