The effects of wogonin on redox status of malignant and normal T cells. (A) Malignant Jurkat T cells produce ROSs at a greater level than normal T cells. The basal redox status of Jurkat, freshly isolated normal peripheral blood T cells (T0), or T cells further cultured for 6 days (T6) were monitored by the oxidation-sensitive fluorescent dyes for ·O2– and H2O2. The unfilled and light gray profiles are normal peripheral blood T0 and T6 cells, respectively. The dark gray profile is Jurkat T cells. The data are also presented as bar charts. (B) Wogonin has stronger effects on the redox status of malignant than normal T cells. T0,T6 and Jurkat T cells were treated with 50 μM wogonin for 60 minutes. The redox status was measured as in panel A. The data are also presented as bar charts. (C-D) Wogonin exerts stronger inhibition of TNFα-induced ROSs in malignant T cells. Normal peripheral blood T (T0) (C) and Jurkat T cells (D) were treated with 100 ng/mL TNFα in the presence or absence of 50 μM wogonin. The ·O2– and H2O2 levels were measured after 2 hours of treatment by the oxidation-sensitive fluorescent dyes as in Figure 2. (E) Data from panels C and D are compared by bar charts. The comparative analysis was carried out in the same experiment on the same day using the same instrument settings. Error bars indicate SD of triplicate experiments.
