Adhesion of PMNs to VWF under flow conditions. Freshly isolated PMNs (5 × 105 cells/mL) were perfused for 6 minutes over VWF-coated coverslips at a shear rate of 50 s–1. (A) Perfusion of nonstimulated PMNs was recorded for 6 minutes, and leukocyte interactions were analyzed to determine transient (less than 3 seconds) contact. Depicted are the numbers of contact for each minute. (B) Perfusion of nonstimulated (○) or PMA-stimulated (•) PMNs was recorded for 6 minutes and was analyzed to determine the amount of stable adherent cells. Shown is the cumulative number of stably adherent cells compared with time of perfusion. Data represent mean ± SEM of 7 to 8 experiments and are corrected for adhesion to PVP-coated coverslips (less than 2 cells/mm2 after 6 minutes).
