Retroviral transduction of AML1-ETO. (A) Schematic diagram of MSCV retroviral constructs (MSCV AML1-ETO IRES EGFP and MSCV IRES EGFP). XbaI, BamHI, and NcoI represent restriction enzyme sites. (B) Western blotting analysis for AML1-ETO expression. Lysates of FACS-sorted GFP-positive BM cells were examined for AML1-ETO fusion proteins by Western blotting analysis. AML1-ETO-expressing Kasumi-1 cells were used as a positive control.