Transplantation assay of the AML1-ETO-transduced BM cells. (A) Flow cytometric analysis of the GFP-positive BM cells from mice that received a transplant of empty-vector-transduced wild-type BM cells (left), empty-vector-transduced WT1-Tg BM cells (second from left), AML1-ETO-transduced wild-type BM cells (second from right), and AML1-ETO-transduced WT1-Tg BM cells (right). (B) Kaplan-Meier plot showing survival of mice after BM transplantation. Empty-vector-transduced wild-type BM cells (no symbol, n = 6), empty-vector-transduced WT1-Tg BM cells (○, n = 8), AML1-ETO-transduced wild-type BM cells (▪,n = 8), and AML1-ETO-transduced WT1-Tg BM cells (•, n = 12). (C) May-Giemsa staining (top) and FACS analysis of GFP expression (bottom) of BM cells from the leukemic mice (original magnification, × 1000). (D) Western blotting analysis of AML1-ETO and WT1 expression. Lysates of the BM cells from the mice that received a transplant of AML1-ETO-transduced wild-type or WT1-Tg BM cells were examined. K562 and Kasmi-1 cells were used as positive controls for WT1 and AML1-ETO, respectively. (E) (Top) Schematic representation of myelopoiesis in the BM from mice that received a transplant of empty-vector-transduced wild-type BM cells, empty-vector-transduced WT1-Tg BM cells, AML1-ETO-transduced wild-type BM cells, and AML1-ETO-transduced WT1-Tg BM cells. (Bottom) May-Giemsa staining of FACS-sorted BM cells from mice that received a transplant of empty-vector-transduced wild-type BM cells. MyBl indicates myeloblasts; Pro, promyelocytes; Mye/Met, myelocytes and metamyelocytes; Band, band-formed neutrophils; and Seg, segment-formed neutrophils. (C,E) Images were visualized and acquired as described in Figure 4B.