β-Like globin gene expression in 5′HS2 core deletion mice. (A) Sample gels of Ey and Bh1 expression in yolk sac primitive erythroid cells. Graph of composite data from assays of Ey and Bh1 mRNA in WT/S and ΔHS2c/S yolk sac. WT values are the WT D/S ratio divided by itself to normalize to 1.0. The mutant/S ratio is divided by the prenormalization WT D/S ratio. Error bars represent the standard deviation of the mean for multiple individual embryos before normalization. (B) Sample gels of Bmaj+min expression in embryonic day 15.5 fetal liver of WT D/S and ΔHS2c/S mice. Graph of composite data from assay of Bmaj+min mRNA in WT D/S and ΔHS2c/S yolk sac (YS), fetal liver (FL), and adult peripheral blood (PB). Data normalization is done as described for Figure 3. Five or more embryos and 3 or more adult mice were assayed. ΔHS2c or ΔHS2core indicates the core deletion.