Effects of TK inhibitors on expression of cell-surface antigens on HMC-1.2 cells. (A) HMC-1.2 cells were exposed to control medium (Co, □), PKC412, 1 μM (▪), AMN107, 1 μM (▨), or imatinib, 1 μM (▦) at 37°C for 24 hours. After incubation, cells were examined for expression of various CD antigens by flow cytometry using CD-specific mAbs. The figure shows the mean fluorescence intensity (MFI) levels as percent of control (Co, □). Results represent the mean ± SD of 3 independent experiments. ^*P < .05 compared with control. (B) Dose-dependent effect of PKC412 on expression of CD63 in HMC-1.2 cells. Cells were incubated with various concentrations of PKC412 as indicated at 37°C for 24 hours. Cells were then examined for expression of CD63 by flow cytometry. As visible, PKC412 dose dependently decreased expression of CD63.