Inhibition of leukemic cell infiltration in bone marrow and spleen by intermittent hypoxia. (A) In the same experiments as those described in Figure 2, cells were isolated from bone marrow (top row) and spleen (bottom row) and suspended in RPMI 1640 medium. Then, scattergram of FSC versus SSC was created in flow cytometry to allow gating on the myeloid cells. R1 and R2 regions indicate myeloid cells, respectively, in BM and in spleen cells. (B) The spleen of every mouse in the same experiments as Figure 2 was weighed and data are shown as the ratio of spleen (mg) to body wt (g). Each column represents the mean of 3 or 4 mice in an independent experiment; error bars represent SD. *P < .001, compared with other groups; #P > .05, compared with mice i and ii. Symbols i to v represent mice with the same treatment as in Figure 2.