Expression of MTAP in primary T-ALL and PBMCs from a healthy donor. Protein was extracted and subjected to Western blot analysis as described in “Materials and methods.” Membrane blots were probed with an MTAP antibody followed by an alkaline phosphatase-conjugated antimouse secondary antibody. Membranes were also probed with a β-actin antibody as a control for protein loading. Proteins were visualized using a STORM 840 Phoshorimager. Protein from MOLT-4 and CEM cell lines were used as a positive and negative control, respectively. MW indicates molecular weight standards.