Selective rescue of normal PBMCs and MTAP⁺ primary T-ALL from L-alanosine toxicity by EFA. Primary T-ALL and PHA-stimulated lymphocytes from a healthy donor were treated with 20 μM and 40 μM L-alanosine, respectively, and with increasing concentrations of EFA for 3 days. Control cells were cultured in media alone. Cells were then pulsed with ³H-thymidine for 6 hours and incorporation of ³H-thymidine was determined in a scintillation counter. All culture conditions were performed in triplicate and error bars represent standard deviation. ▴ indicates normal PBMCs; •, MTAP⁺ T-ALL patient 061219; and ▵, MTAP^- T-ALL patient 122614-R (relapsed patient).