Ki-67, FoxP3, and CTLA-4 expression in treated macaques. Flow cytometry analysis of the Ki-67 marker on CD4⁺ or CD8⁺ T cells at the time of ART initiation (week 0) and 1 week after MDX-010 inoculation (week 6) in blood (A) or lymph nodes (B). FoxP3 expression measured by FACS in CD25⁺CD4⁺ T cells and CD25⁺CD8⁺ T cells in blood at weeks 4 and 13 (C). (D) Level of FoxP3 and CTLA-4 expression in lymph nodes of macaques before treatment (time 0) and 1 week after CTLA-4 treatment (week 6). For panels A-C, mean values plus standard error are shown. For panel D, horizontal bars within boxes correspond to the median; box limits correspond to the 25th and 75th percentiles; and vertical lines extend to the 10th and 90th percentiles.