NK cells develop in the absence of PLC-γ2. (A) Representative FACS profile of WT and Plcg2^-/- splenocytes gated on the lymphoid population. Numbers in plots represent means of NK counts in 19 WT and 19 Plcg2^-/- individual mice. (B) Transcripts of activating receptors, adaptors, and effector molecules in IL-2-activated splenocytes (lymphokine-activated killer [LAK] cells), and resting CD3^-NK1.1⁺ purified NK cells from WT or Plcg2^-/- mice. (C) Fresh splenocytes were stained with the indicated markers. Gated CD3^- NK1.1⁺ cells are shown. Percentages in the histograms indicate cells staining positive for the specific marker. (D) Mean fluorescent intensity (MFI) is shown for the indicated markers expressed on gated CD3^-NK1.1⁺Plcg2^-/- splenocytes. Bars represent percentage values of MFI found on gated CD3^-NK1.1⁺ WT splenocytes.