Figure 1.
Identification of SI-CLP as stabilin-1 interacting protein. (A) Schematic representation of stabilin-1 fragment used as a bait in yeast 2-hybrid screening. (B) GST pull-down assay using bacterially expressed GST-P9 (lane 1), GST (lane 2), and GST-F7 (lane 3). 35S-labeled full-length SI-CLP specifically interacts with P9 fragment and fasciclin 7 domain of stabilin-1. (C) Control of GST-fused protein amounts used in pull-down reaction.