Confirmation of AML cell growth in NOD/SCID mice. Ten million cells were injected into NOD/SCID mice and marrows were analyzed for human myeloid cell content 6 weeks later. (A) Diagnostic peripheral blood smear from AML-M2 patient 10. Cells were stained with hematoxylin and eosin (H&E) before analysis at 400 × magnification via a 40 ×/0.75 objective lens. (B) Murine marrow that was injected with cells from the same AML-M2 patient as in panel A. Myeloblasts, featuring Auer rods (arrow), are present, indicating AML. Cells were stained with H&E before analysis at 1000 × magnification via a 100 ×/1.3 objective lens. (C) Dual fusion, dual-color fluorescent in situ hybridization of a relapsed t[8,21] AML-M2 sample. Cells positive for the rearrangement exhibit 1 green, 1 red, and 2 orange spots. (D-F) Examples of NOD/SCID engrafted, FACSorted CD33⁺/CD45⁺ cells exhibiting AML-M2 t[8,21] rearrangement. Pictures of blood smears were taken on a Leica DFC 300F camera.