EKLF plays a global role as a transcriptional activator in vivo. (A) Scatter plot of gene-expression profiling from EKLF^+/+ and EKLF^-/- E14.5 fetal livers. Green dots indicate genes that have been downregulated when EKLF is absent; red dots, genes that have been upregulated when EKLF is absent; and yellow dots, genes that are not significantly altered. (B) Relative expression levels of putative EKLF target genes in E14.5 EKLF^-/- fetal livers compared with EKLF^+/+. Levels were measured by real-time PCR and normalized to hypoxanthine phosphori-bosyltransferase (HPRT). Data are presented as mean ± SEM, n = 3. (C) Scatter plot of gene expression from the control EKLF-ER cell line B1.6 and tamoxifen-treated cells. (D) Relative expression levels of putative EKLF target genes in tamoxifen-treated B1.6 cells compared with control. (E) Venn diagram of the overlap (yellow) between EKLF-activated genes in tamoxifen-treated B1.6 cells (green) and EKLF-activated genes in fetal liver (red). (F) Representative Western blot of dematin and AHSP expression in whole-cell extracts from E14.5 fetal livers. Actin is presented as a loading control.