Histopathology of AOD and EAP in d3tx B6AF1 mice and comparison of suppression of the 2 autoimmune diseases by Tregs. (A) Prostatitis with lymphocytic infiltration in a d3tx mouse. (B) Normal anterior prostate of a d3tx mouse suppressed with 3 × 105 male Tregs. (D) Severe oophoritis in a d3tx female mouse, with heavy inflammatory infiltrate and oocyte loss. (E) Normal ovary of a d3tx mouse suppressed by 5 × 105 Tregs from normal female donors. Original magnification × 400 (A-B) and × 200 (D-E); hematoxylin and eosin. Histological studies were done using an Olympus BH2 microscope (Olympus, Melville, NY), and pictures were taken with an Olympus DP12 digital microscope camera. Images were taken with 20×/__ NA (panels __) and 40×/__ NA (panels __) objectives. (C) The cell dose response of EAP suppression by Tregs from male (□), female (○), and NOr male (▵) donors. (F) The cell dose response of AOD suppression by Tregs from male (□) and female (○) donors.
