Figure 4.
Figure 4. Histopathologic illustration of MALT/MZ lymphomas. (A) Low-power view (× 50) of the lymphoid infiltration in a gastric biopsy sample tissue section stained with hematoxylin and eosin from a patient with H pylori–associated gastric MALT lymphoma. The arrow shows a germinal center surrounded by an enlarged marginal zone infiltrating the gastric lamina propria. (B) High-power view (× 400) of an intestinal biopsy sample tissue section stained with hematoxylin and eosin from a patient with C jejuni–associated IPSID, revealing centrocyte-like cells (CCLs) infiltrating the crypt epithelium and forming lymphoepithelial lesions (LELs). (C) Sections of jejunum (× 100) stained with primary antibodies directed against the B-cell marker CD20 (appears brown when stained with enzyme-linked secondary antibodies) and counterstained with hematoxylin show CD20+ centrocyte-like lymphocytes pervading the lamina propria surrounding crypts. (The top inset shows a higher power view of the epithelium.) CD20+ CCLs infiltrate the crypt epithelium and produce characteristic LELs (arrow). (D) High-power view (× 400) of a gastric biopsy sample tissue section stained according to the Giemsa technique from a patient with H pylori –associated gastric MALT lymphoma. The gastric mucosa is heavily infected by H pylori (arrow). (E) Immunohistochemical analysis of a jejunal section (× 400) from a patient with IPSID and stained with an anti–C jejuni monoclonal antibody (brown) and hematoxylin. The arrows point to immunolabeled material shown at a higher magnification in the top-right inset. The top-left inset shows a crypt section with intraluminal immunolabeled bacteria. (F) A blood smear (× 1000) colored according to the May-Grünwald-Giemsa technique showing a typical villous lymphocyte from a patient with HCV-associated splenic lymphoma with villous lymphocytes. Sections were viewed using a Zeiss Axiophot microscope (Carl Zeiss, Thornwood, NY) and Olympus UPlan F1 5 ×/0.12 numeric aperture (NA) (A), 40 ×/0.7 NA (B, D, E), 10 ×/0.30 (C), and 100 ×/0.30 NA (F; oil immersion) objectives (Olympus, Melville, NY), and were photographed using a Spot RT digital camera and Spot 3 software (both from Diagnostic Instruments, Sterling Heights, MI).

Histopathologic illustration of MALT/MZ lymphomas. (A) Low-power view (× 50) of the lymphoid infiltration in a gastric biopsy sample tissue section stained with hematoxylin and eosin from a patient with H pylori–associated gastric MALT lymphoma. The arrow shows a germinal center surrounded by an enlarged marginal zone infiltrating the gastric lamina propria. (B) High-power view (× 400) of an intestinal biopsy sample tissue section stained with hematoxylin and eosin from a patient with C jejuni–associated IPSID, revealing centrocyte-like cells (CCLs) infiltrating the crypt epithelium and forming lymphoepithelial lesions (LELs). (C) Sections of jejunum (× 100) stained with primary antibodies directed against the B-cell marker CD20 (appears brown when stained with enzyme-linked secondary antibodies) and counterstained with hematoxylin show CD20+ centrocyte-like lymphocytes pervading the lamina propria surrounding crypts. (The top inset shows a higher power view of the epithelium.) CD20+ CCLs infiltrate the crypt epithelium and produce characteristic LELs (arrow). (D) High-power view (× 400) of a gastric biopsy sample tissue section stained according to the Giemsa technique from a patient with H pylori –associated gastric MALT lymphoma. The gastric mucosa is heavily infected by H pylori (arrow). (E) Immunohistochemical analysis of a jejunal section (× 400) from a patient with IPSID and stained with an anti–C jejuni monoclonal antibody (brown) and hematoxylin. The arrows point to immunolabeled material shown at a higher magnification in the top-right inset. The top-left inset shows a crypt section with intraluminal immunolabeled bacteria. (F) A blood smear (× 1000) colored according to the May-Grünwald-Giemsa technique showing a typical villous lymphocyte from a patient with HCV-associated splenic lymphoma with villous lymphocytes. Sections were viewed using a Zeiss Axiophot microscope (Carl Zeiss, Thornwood, NY) and Olympus UPlan F1 5 ×/0.12 numeric aperture (NA) (A), 40 ×/0.7 NA (B, D, E), 10 ×/0.30 (C), and 100 ×/0.30 NA (F; oil immersion) objectives (Olympus, Melville, NY), and were photographed using a Spot RT digital camera and Spot 3 software (both from Diagnostic Instruments, Sterling Heights, MI).

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