Cytoskeleton structure of Rac1^–/–;Rac2^–/– erythrocytes. (A) Erythrocytes fixed with acrolein and stained with rhodamine-phalloidin for F-actin. Arrowheads in magnified inset show meshwork gaps and aggregates of F-actin in Rac1^–/–;Rac2^–/– erythrocytes. (B) RBCs stained with anti–band 3 antibody to visualize band 3 distribution. Images were obtained with a × 63 oil-immersed objective lens, software zoom × 2, numerical aperture 1.4; 1 unit represents 7.3 μm.