Figure 5.
LAK cells generated in the presence of anti-2B4 or anti-CD48 mAb, but not anti-CD2 mAb, do not make IFN-γ when mixed with RMA/S targets. WT LAK cells were generated in the presence or absence of 5 μg/mL mAbs indicated in the figure. (A) LAK cells (1 × 105) were mixed with RMA/S cells at 1:1 ratio, and IFN-γ production was measured by FACS using anti–IFN-γ-PE, as described in “Materials and methods.” (B) LAK cells were treated with 50 ng/mL phorbol myristate acetate (PMA) and 500 ng/mL ionomycin and analyzed for IFN-γ production by FACS. The numbers in the top left quadrant indicate the percentage of NK cells expressing IFN-γ. The results of this particular experiment represent those from 5 other independent experiments.
