Quantitation of CYTIP mRNA expression in DC populations. RNA from CD14⁺ monocyte-derived immature DCs (iDCs) and mature DCs (mDCs), CD34⁺ precursor-derived immature and mature DCs, and emigrated Langerhans cells (LCs) and dermal cells from skin explant cultures (dDCs) was collected and subjected to quantitative reverse transcriptase (RT)-PCR using CYTIP-specific primers and probes. Values are relative to 18S RNA. CYTIP mRNA is expressed in a variety of DCs and induced on maturation.