Cellular CYTIP distribution in response to stimulation of surface molecules by antibodies. (A) Mature monocyte-derived DCs were allowed to bind to antibodies immobilized on glass slides for 20 to 30 minutes. After fixation in acetone, CYTIP was visualized by a specific monoclonal anti-CYTIP antibody detected by Alexa 488 (green fluorescence). DAPI was used to visualize the nuclei (blue fluorescence) and PKH26 to mark the plasma membrane (red fluorescence). Images were obtained by confocal microscopy. White lanes were used to perform line scans shown in panel B. (B) Red lines show pixel intensities for PKH26; green lines show pixel intensities for CYTIP staining. On binding to fibronectin, and antibody-mediated binding of CD18, ICAM-1 (CD54), and VCAM-1 (CD106), but not to poly-L-lysine and anti-CD11a, -CD11c, and -VLA-4 (CD49d), CYTIP moves toward the membrane in DCs.