Silencing of CYTIP is specific and does not interfere with the expression or induction of other molecules. (A) CYTIP expression was monitored 48 hours after silencing by Western blotting and intracellular FACS analysis. The expression level can be kept to the level of immature DCs and was achieved in 60% to 90%. iDC indicates immature DC; mDC, mature DC; simDC, mature DC silenced for CYTIP; and scramble, unrelated control siRNA used in silencing procedure. (B) Expression of other molecules known to be induced or up-regulated during maturation of DCs as well as adhesion structures (CD40, CD80, CD83, MHCII, CD11a, CD11b, CD11b, CD11c, CD18, CD54, CD49d, CD49e, and CD106) are not influenced by the silencing procedure, as shown by FACS analysis. Broken line indicates isotype control; solid line, immature DC; bold line, mDC silenced for CYTIP; and dotted line, mature DC.