DCs adherence to fibronectin is CYTIP dependent. CYTIP was specifically silenced in DCs during maturation. DCs were labeled with calcein and allowed to adhere to fibronectin-treated 96-well plates. Adherence to fibronectin was measured by quantifying calcein emission. Mature DCs (mDCs) adhere less efficiently to fibronectin than immature DCs (iDCs). Silencing of CYTIP partially restores adherence in mature DCs (simDCs). The unrelated siRNA (scramble) does not change the adhesion features of mDCs.