DC adherence to T cells but not to endothelial cells depends on CYTIP. T cells were applied to poly-L-lysine-coated 96-well plates, and endothelial cells were cultured in 96-well plates. Calcein-labeled DCs were allowed to adhere to T cells or endothelial cells for 45 minutes. Adherent cells were quantified by measuring calcein emission. CYTIP silencing in mature DCs (simDCs) enhanced binding to T cells (A) but did not alter binding activities of DCs to endothelial cells (B). iDC indicates imature DC; mDC, mature DC.