Cellular localization of CYTIP in DCs in response to T-cell contact. (A) DCs were allowed to adhere to poly-L-lysine immobilized bulk T cells for the indicated time points. CYTIP was visualized by immunostaining (green fluorescence). Nuclei are stained with DAPI (blue fluorescence). Cells were visualized with a confocal microscope. Accumulation of CYTIP in the contact zone of DCs and T cells can be observed starting at 15 minutes with a maximum at 30 minutes. (B) Percentage of contact sites with polarized CYTIP from all DCs with T-cell contact was calculated in 3 scans each of 0.546 mm² for each time point.