Strategy used for evaluation of transgene expression in different cell lineages by flow cytometry. A representative peripheral blood analysis of dog 1655 at 4 months after treatment is shown. (A) Scattergram of forward and side scatter (SSC) of peripheral blood showing gate R1, lymphocytes; gate R2, monocytes; and gate R3, granulocytes. (B) GFP⁺ cells in lymphocyte gate. (C) GFP⁺ cells in monocyte gate. (D) GFP⁺ cells in granulocyte gate. (E) GFP⁺ T (CD3⁺) cells in lymphocyte gate. (F) GFP⁺ B (CD21⁺) cells in lymphocyte gate.