Figure 6.
Polyclonal T-cell reconstitution following intravenous gene therapy. (A) T-cell diversity in dog 1547 6 months following gene therapy as determined by TCR Vβ CDR3 spectratyping for 12 distinct TCR Vβ segments. (B) Assessment of vector integration sites by LAM-PCR from genomic DNA from CD3+ cells from dog 1547 (lanes 2-4) and dog 1551 (lanes 5-7) 5 months following gene therapy. Lane 1, DNA molecular weight (MW) marker; lane 8, H2O control. → indicates size of final vector control band with incomplete digestion by PvuII.
