Venous pressure elevation maneuvers.. Capillary filtration coefficient (g/[min × 100 g lung weight. × ΔPc]) and challenge-induced weight gain (g/g lung weight) are shown in the absence or presence of anti-HNA-2a. Following an isogravimetric steady-state phase, venous pressure elevations were performed at indicated time points. Neutrophils derived from either high-expressing (A,C) or low-expressing (B,D) individuals were administered following the first hydrostatic challenge at minute 15. Anti-HNA-2a (black squares) or control antibody (open circles) was added at minute 50. After administration of CD177 high-expressing granulocytes and anti-HNA-2a, TRALI develops after the sixth pressure elevation (A), whereas no significant changes occur after administration of low-expressing granulocytes and anti-HNA-2a (B). Addition of fMLP to the buffer fluid at minute 120 (C,D) promotes TRALI induction in the presence of either high-expressing (C) or low-expressing (D) neutrophils. All results are presented as mean values + SD for 5 independent experiments. Note the discontinuous y-axis for. K_f,c. Pc denotes capillary pressure.